Police stopped the van on Friday morning on the M5 near Cullompton, Devon following reports a group of people were seen getting into the back of the van after arriving by boat.
All the people in the van are believed to be Vietnamese. Police were called just before 7:10am on Friday to Newlyn following up reports about the group of people getting into a van.
Frank Walling, 72, from Colne, Jon Ransom, 63, from Kent, Keith Royston Plummer, 62, from Sheerness, and Glen Martin Bennett, 55, from Burnley were charged with human trafficking under the Modern Slavery Act.
They were also charged with assisting unlawful immigration to a member state.
The men are remanded in custody and will appear before Truro Magistrates tomorrow.
Devon and Cornwall Police said: “The van and another vehicle were stopped by officers on the M5 near J28 just after 9am in which 29 men, women and children were located, all believed to be Vietnamese; Police and partners continue to take care of those located in the van.”
A British Red Cross spokesperson said volunteers were supporting the 29 alleged victims of human trafficking.
They said: “Our volunteers are continuing to support a number of people who are the alleged victims of human trafficking.
“We are providing them with practical and emotional support at a rest centre while the police conduct their investigations.”
A spokesman for Devon Children and Families Partnership said: “The UK Government will decide what the next steps will be for these individuals.
“In the meantime, Devon and Cornwall agencies are supporting the group, and arrangements for temporary accommodation will made if any of the individuals are under 18 years of age, pending further decision about their status.”
The National Crime Agency, Border Force and Immigration Enforcement an other relevant partner agencies are assisting police as enquiries continue.