Juventus drew 1-1 with Ajax in Amsterdam to place their quarter-final tie finely in the balance.
Ronaldo had earlier given Juventus the lead with a fine header but his effort at was cancelled out by David Neres.
While Juve will go into the second leg as favourites to progress to the last four, they were second best for large periods at the Johan Cruyff Arena as De Jong ran the game from midfield.
He racked up a whopping 117 touches in the game and showed why Barcelona were happy to fork out £65million to sign him in the summer.
However, Ronaldo was left furious art Juve’s inability to press him during a vital moment of the match.
As the Nou Camp-bound De Jong embarked on a trademark burst from deep, he was seemingly given the freedom of the pitch as both Blaise Matuidi and Rodrigo Bentancur backed away from him.
Ronaldo could be seen waving his arms as he tried to encourage them to close him down.
At a loss with the situation, Ronaldo turned round in a fit of rage and appeared to shout into the air.
Juve manager Massimiliano Allegri admitted after the game that De Jong had caused his side problems all night.
“De Jong is the real source of their game,” the Italian said.
“We tried to control him with (Rodrigo) Bentancur in the early stages but, when he understood that, he moved deeper, and the forwards had to work hard on him. He played very well.
“Ajax have great qualities. They keep the ball well even when there are no spaces.
“I think we defended well even if we had to be better when we won the ball in midfield.”
Ajax were left to rue a number of missed opportunities as they notched up 19 shots with an impressive six on target.
However, they have proved they can perform on the biggest stage under age pressure after turning around a 2-1 defeat by Real Madrid in the last round by winning 4-1 at the Bernabeu in the second.
And manager Erik ten Hag has urged them to draw on that experience by spring another upset.
“One-one is not the result you hope for, but it is a result,” he said.
“Last time we lost 2-1 to Madrid at home, so we are still in it. We had some very good opportunities.”